2010 HIV Diagnostics Conference

This agenda is still in development and is posted for planning purposes only. Presentation titles and speakers have not yet been finalized.

Wednesday, March 24

8:00am-9:30am Opening Session

Moderator – Mark Pandori, PhD, San Francisco Department of Public Health, HIV Diagnostics Conference Planning Committee Chair

Evolution of HIV Diagnostics and Goals of the 2010 Conference

Bernard Branson, MD, Centers for Disease Control and Prevention

HIV Testing in Public Health Laboratories: Results from the 2009 APHL HIV Testing Practices Survey

Barbara Werner, PhD, Massachusetts Department of Health

Model Performance Evaluation Program (MPEP) HIV Rapid Testing Survey: Report of Sample Shipment Results, September 2009

Steven Ethridge MT(ASCP), Centers for Disease Control and Prevention

10:00am-11:30am Rapid Testing Algorithms at the Point of Care/Contact

The presentations in this session describe the implementation of rapid multi-test algorithms at the point-of-contact and evaluate the performance of these algorithms in diverse settings.

Moderator – Kevin Delaney, MPH, Centers for Disease Control and Prevention

Lessons Learned from San Francisco’s Experience with Rapid Testing Algorithms

Thomas Knoble, MSW, San Francisco Department of Public Health

Two-Rapid Test Strategy in Anonymous HIV Counseling and Testing (ACT) Sites in New York State 2008-2009

April Richardson-Moore, RN, MPH, New York State Department of Health

Comparison of multiple laboratory-performed rapid HIV tests with standard confirmatory assays for assessment of multi-rapid test algorithms

Linda Styer, PhD, Wadsworth Center, New York

Statewide Implementation of Rapid-Rapid Testing in New Jersey

Eugene G. Martin, PhD, UMDNJ – Robert Wood Johnson Medical School

11:30am-1:00pm Special Lunch Session – Sponsored by Abbott

Evolution in Real-Time: The Challenge of HIV-1 Diversity and Implications for Assay Performance

John Hackett Jr., PhD, Research Fellow, Volwiler Society; Manager, Emerging Pathogens and Virus Discovery Programs, Abbott Diagnostics, Abbott Park, IL

1:00pm-2:30pm Rapid Testing Algorithms at the Point of Care/Contact

This session will continue the discussion of rapid testing algorithms and address other issues in POC testing including expanded testing programs, alternative uses of rapid tests, and new POC testing technologies.

Moderator – Michael Pentella, PhD, D(ABMM), University of Iowa Hygienic Laboratory

Use of a three rapid HIV test algorithm at point-of-care settings to facilitate same day receipt of a final HIV test result, referral to medical care, and address false-positive screening tests

Jacqueline Rurangirwa, MPH, County of Los Angeles, Department of Public Health, Office of AIDS Programs and Policy

Examination of an alternative algorithm for HIV diagnostics using HIV-1/2 rapid tests as confirmatory assays

Silvina Masciotra, MS, Centers for Disease Control and Prevention

Expanded HIV Rapid Testing in Emergency Departments (ED) – Chicago, Illinois 2007-2008

Nancy R. Glick, MD, Mt. Sinai Hospital, Chicago, IL

Multiplexed POC Device for HIV and Co-Infection Serodiagnosis

Michael Lochhead, PhD, mBio Diagnostics , Inc.

3:00pm-4:30pm Bridging Point-of-Care/Contact & Laboratory Testing

The session will focus on the interface between POC and laboratory HIV testing. It will highlight innovative collaborations to improve quality assurance, discuss the implications of alternative testing algorithms for surveillance, and introduce a novel proposal to link POC and laboratory testing in a single diagnostics algorithm.

Moderator – Kathleen Krchnavek, MSSW, Wisconsin Department of Health

Establishing Ongoing Competency Assessment to Maintain HIV Testing Skills

Teri Dowling, MA, MPH, San Francisco Department of Public Health

A Successful HIV Testing Quality Assurance Program; New York State Experience

Mara L. San Antonio-Gaddy, RN, MSN, New York State Department of Health

The Potential Effect of Alternative Algorithms on HIV Surveillance

Kristen Mahle, MPH, Centers for Disease Control and Prevention

The HIV Bridge Algorithm: Linking Point-of-Care, Laboratory and Patient Care

Berry Bennett, MPH, Florida Bureau of Laboratories

Thursday, March 25

8:00am-9:30am Serologic Testing Methods

The session will focus on HIV serology, from the analysis of screening immunoassay data to a comparison of confirmatory methods. Challenges like false positives, indeterminate results, and HIV-2 will also be discussed.

Moderator – William A. Meyer III, PhD, D(ABMM), Quest Diagnostics

Using all the data: Immunoassay signal-to-cutoff values provide useful information that should be considered in HIV diagnostic algorithms

Kevin P. Delaney, MPH, Centers for Disease Control and Prevention

False-positive HIV Test Results Using a Peptide-based Enzyme Immunoassay (EIA) in Pregnant Women and Others

Laura G. Wesolowski, PhD, Centers for Disease Control and Prevention

Dramatic Reduction in Indeterminate Results from HIV Antibody Testing of Blood Donors by Conversion from Western Blot to an Immunofluorescence Assay

Michael P. Busch, MD, PhD, Blood Systems Research Institute and UCSF

Use of Multispot (MS) HIV-1/HIV-2 Rapid Test to confirm HIV-1/HIV-2 Plus O Enzyme immunoassay (EIA) results shorten reporting time for HIV testing and identify cryptic HIV-2 infection

Robert W. Coombs, MD, PhD, FRCPC, University of Washington

10:00am-11:30am Testing for Recent HIV Infection

This session will include an overview of HIV incidence testing followed by presentations of novel methods and technologies for the detection of recent HIV infection.

Moderator – S. Michele Owen, PhD, Centers for Disease Control and Prevention

Recent advances in development and application of assays/algorithms for detection of recent HIV infections and estimation of incidence

Michael P. Busch, MD, PhD, Blood Systems Research Institute and UCSF

Optimization and Calibration of Less Sensitive and Avidity Modified Protocols for the VITROS Immunodiagnostic Products Anti-HIV1+2 assay for Detection of Early HIV Infections and Incidence Estimation

Sheila M. Keating, PhD, MSPH, Blood Systems Research Institute

Antibody avidity-based assay for identifying recent HIV-1 infections based on Genetic Systems^TM 1/2 plus O EIA

Silvina Masciotra, MS, Centers for Disease Control and Prevention

IgG3 as a Biomarker for Distinguishing Recent from Established HIV-1 Infection

Kelly A. Curtis, PhD, Centers for Disease Control and Prevention

1:00pm-2:30pm Fourth Generation Immunoassays

In this session, clinical data as well as laboratory evaluations and assay comparisons will be presented for fourth generation antigen/antibody combination immunoassays.

Moderator – Bernard Branson, MD, Centers for Disease Control and Prevention

Performance of the AxSym Ag/Ab HIV Combo assay, a 4^th generation assay for routine HIV screening

Christiane Claessens, MSc, INSPQ/LSPQ, Sainte-Anne-de-Bellevue, QC, Canada

Performance Evaluation of the Bio-Rad GS HIV Combo Ag/Ab EIA

Kathleen Shriver, PhD, Bio-Rad Laboratories

Development* of an HIV-1/2/O Antigen-Antibody Combo Assay for Use on Ortho Clinical Diagnostics VITROS® Integrated and Immunodiagnostic Systems

Patrick Kilmartin, B.Sc., M.Sc., Ortho Clinical Diagnostics

Clinical and Analytical Evaluation of the ARCHITECT®HIV Ag/Ab Combo Assay

Barbara A. Kaesdorf, MS, MT(ASCP), CCRA, Abbott Diagnostics

Assessing the sensitivities of laboratory-based and point-of-care HIV antigen-antibody combination tests using a panel of specimens from recently and acutely infected individuals

Mark Pandori, PhD, San Francisco Department of Public Health

3:00pm-4:30pm Applications of Nucleic Acid Testing Technologies

This session will present current research in nucleic acid testing, from evaluations of NAAT for screening nontraditional specimen types to its use as a confirmatory test. Additionally, new technologies for POC nucleic acid testing will be introduced.

Moderator – Sally Liska, DrPH, San Francisco Department of Public Health

Optimization of Abbott m2000 RealTime HIV-1 Viral Load Assay on Breastmilk, Dried Blood Spots, Genital Secretions, Cerebrospinal Fluid, Urine, and Throatwash

Julie A. E. Nelson, PhD, University of North Carolina at Chapel Hill

Evaluating Kit-Based HIV-1 DNA PCR Protocol for Confirming Infection

Carolyn D. Dawson, BS, Centers for Disease Control and Prevention

TBD

James Bremer, PhD, Rush University

Point-of-Care Molecular Diagnostics

Marco Schito, PhD, Henry Jackson Foundation, DAIDS, NIH, Bethesda, MD

Friday, March 26

8:00am-9:30am New Approaches in HIV Testing

This session will highlight novel methods in prevention and recent advances in diagnostic testing technology.

Moderator – Steven Ethridge, MT(ASCP), Centers for Disease Control and Prevention

ELISA Strength of Signal in a Protocol to Prevent Mother-to-Child HIV Transmission

Michael Loeffelholz, PhD, ABMM, University of Texas Medical Branch

Rapid Detection of HIV-1 p24 Antigen and of Antibodies to HIV-1 and HIV-2 Using Magnetic Immunochromatography

Timothy C. Granade, MS, Centers for Disease Control and Prevention

Europium Nanoparticle-based Immunoassay (ENIA) for Sensitive and Early Detection of Human Immunodeficiency Virus Type 1 Capsid (p24) Antigen

Shixing Tang, MD, PhD, Food and Drug Administration

A novel method of creating molecular control used in the NLHRS HIV-2 Viral Load Assay and subsequent results in a NIBSC-WHO study to evaluate candidate international standards for HIV-2 RNA

John Kim, PhD, National Laboratory for HIV Reference Services, Public Health Agency of Canada

10:00am-11:30am Concluding Session

Conference Wrap-Up and Next Steps

Moderated by Bernard Branson, MD, Centers for Disease Control and Prevention

Preliminary Program