2010 HIV Diagnostics Conference

Opening Session

Moderator – Mark Pandori, PhD, San Francisco Department of Public Health, HIV Diagnostics Conference Planning Committee Chair

Evolution of HIV Diagnostics and Goals of the 2010 Conference

Bernard Branson, MD, Centers for Disease Control and Prevention

HIV Testing in Public Health Laboratories: Results from the 2009 APHL HIV Testing Practices Survey

Barbara Werner, PhD, Massachusetts Department of Health

Model Performance Evaluation Program (MPEP) HIV Rapid Testing Survey: Report of Sample Shipment Results, September 2009

Steven Ethridge MT(ASCP), Centers for Disease Control and Prevention

Rapid Testing Algorithms at the Point of Care/Contact

Moderator – Kevin Delaney, MPH, Centers for Disease Control and Prevention

Lessons Learned from San Francisco’s Experience with Rapid Testing Algorithms

Thomas Knoble, MSW, San Francisco Department of Public Health

Two-Rapid Test Strategy in Anonymous HIV Counseling and Testing (ACT) Sites in New York State 2008-2009

April Richardson-Moore, RN, MPH, New York State Department of Health

Comparison of multiple laboratory-performed rapid HIV tests with standard confirmatory assays for assessment of multi-rapid test algorithms

Linda Styer, PhD, Wadsworth Center, New York

Statewide Implementation of Rapid-Rapid Testing in New Jersey

Eugene G. Martin, PhD, UMDNJ – Robert Wood Johnson Medical School

Rapid Testing Algorithms at the Point of Care/Contact

Moderator – Michael Pentella, PhD, D(ABMM), University of Iowa Hygienic Laboratory

Use of a three rapid HIV test algorithm at point-of-care settings to facilitate same day receipt of a final HIV test result, referral to medical care, and address false-positive screening tests

Jacqueline Rurangirwa, MPH, County of Los Angeles, Department of Public Health, Office of AIDS Programs and Policy

Examination of an alternative algorithm for HIV diagnostics using HIV-1/2 rapid tests as confirmatory assays

Silvina Masciotra, MS, Centers for Disease Control and Prevention

Expanded HIV Rapid Testing in Emergency Departments (ED) – Chicago, Illinois 2007-2008

Nancy R. Glick, MD, Mt. Sinai Hospital, Chicago, IL
Karen Reitan, MA, Public Health Institute of Metropolitan Chicago

Multiplexed POC Device for HIV and Co-Infection Serodiagnosis

Michael Lochhead, PhD, mBio Diagnostics , Inc.

Bridging Point-of-Care/Contact & Laboratory Testing

Moderator – Kathleen Krchnavek, MSSW, Wisconsin Department of Health

Establishing Ongoing Competency Assessment to Maintain HIV Testing Skills

Teri Dowling, MA, MPH, San Francisco Department of Public Health

A Successful HIV Testing Quality Assurance Program; New York State Experience

Mara L. San Antonio-Gaddy, RN, MSN, New York State Department of Health

The Potential Effect of Alternative Algorithms on HIV Surveillance

Kristen Mahle, MPH, Centers for Disease Control and Prevention

The HIV Bridge Algorithm: Linking Point-of-Care, Laboratory and Patient Care

Berry Bennett, MPH, Florida Bureau of Laboratories

Serologic Testing Methods

Moderator – William A. Meyer III, PhD, D(ABMM), Quest Diagnostics

Using all the data: Immunoassay signal-to-cutoff values provide useful information that should be considered in HIV diagnostic algorithms

Kevin P. Delaney, MPH, Centers for Disease Control and Prevention

False-positive HIV Test Results Using a Peptide-based Enzyme Immunoassay (EIA) in Pregnant Women and Others

Laura G. Wesolowski, PhD, Centers for Disease Control and Prevention

Dramatic Reduction in Indeterminate Results from HIV Antibody Testing of Blood Donors by Conversion from Western Blot to an Immunofluorescence Assay

Michael P. Busch, MD, PhD, Blood Systems Research Institute and UCSF

Use of Multispot (MS) HIV-1/HIV-2 Rapid Test to confirm HIV-1/HIV-2 Plus O Enzyme immunoassay (EIA) results shorten reporting time for HIV testing and identify cryptic HIV-2 infection

Robert W. Coombs, MD, PhD, FRCPC, University of Washington

Testing for Recent HIV Infection

Moderator – S. Michele Owen, PhD, Centers for Disease Control and Prevention

Recent advances in development and application of assays/algorithms for detection of recent HIV infections and estimation of incidence

Michael P. Busch, MD, PhD, Blood Systems Research Institute and UCSF

Optimization and Calibration of Less Sensitive and Avidity Modified Protocols for the VITROS Immunodiagnostic Products Anti-HIV1+2 assay for Detection of Early HIV Infections and Incidence Estimation

Sheila M. Keating, PhD, MSPH, Blood Systems Research Institute

Antibody avidity-based assay for identifying recent HIV-1 infections based on Genetic Systems TM 1/2 plus O EIA

Silvina Masciotra, MS, Centers for Disease Control and Prevention

IgG3 as a Biomarker for Distinguishing Recent from Established HIV-1 Infection

Kelly A. Curtis, PhD, Centers for Disease Control and Prevention

A Global Perspective: International Laboratory Poster Highlights

Moderators – Kenneth Landgraf, MS & Tony Tran, MPH, MT(ASCP), Association of Public Health Laboratories

Evaluation of Dried Blood Spots (DBS) for Human Immunodeficiency Virus (HIV-1) Drug Resistance Testing

Dawit Assefa, Ethiopian Health & Nutrition Research Institute

HIV EQAS - Novel Perspectives

Elizabeth Dax, National Serology Reference Laboratory, Australia and Indonesian Collaborators

SMARTube as a Test for Recent Infection

Reshma Kassanjee, University of the Witwatersrand, South Africa

Comparison of Dried Tube and Plasma Specimens in an External Quality Assessment Scheme and Evaluation of Quality of Rapid Testing Performed by Non-Laboratory Staff in Lesotho

Mathabo Lebina, Laboratory Services, Ministry of Health and Social Welfare, Lesotho

The High Positive Immunoassay Confirms HIV-1 Infection and Avoids the Need of Confirmatory Tests

Rodolfo Ochoa-Jimenez, Regional Hospital of Colima, Mexico

Fourth Generation Immunoassays

Moderator – Bernard Branson, MD, Centers for Disease Control and Prevention

Performance of the AxSym Ag/Ab HIV Combo assay, a 4th generation assay for routine HIV screening

Christiane Claessens, MSc, INSPQ/LSPQ, Sainte-Anne-de-Bellevue, QC, Canada

Performance Evaluation of the Bio-Rad GS HIV Combo Ag/Ab EIA

Kathleen Shriver, PhD, Bio-Rad Laboratories

Development* of an HIV-1/2/O Antigen-Antibody Combo Assay for Use on Ortho Clinical Diagnostics VITROS® Integrated and Immunodiagnostic Systems

Patrick Kilmartin, B.Sc., M.Sc., Ortho Clinical Diagnostics

Clinical and Analytical Evaluation of the ARCHITECT®HIV Ag/Ab Combo Assay

Barbara A. Kaesdorf, MS, MT(ASCP), CCRA, Abbott Diagnostics

Assessing the sensitivities of laboratory-based and point-of-care HIV antigen-antibody combination tests using a panel of specimens from recently and acutely infected individuals

Mark Pandori, PhD, San Francisco Department of Public Health

Applications of Nucleic Acid Testing Technologies

Moderator – Sally Liska, DrPH, San Francisco Department of Public Health

Optimization of Abbott m2000 RealTime HIV-1 Viral Load Assay on Breastmilk, Dried Blood Spots, Genital Secretions, Cerebrospinal Fluid, Urine, and Throatwash

Julie A. E. Nelson, PhD, University of North Carolina at Chapel Hill

Evaluating Kit-Based HIV-1 DNA PCR Protocol for Confirming Infection

Carolyn D. Dawson, BS, Centers for Disease Control and Prevention

New HIV-1 RNA Assay Validation: Roche COBAS TaqMan and Abbott RealTime

James Bremer, PhD, Rush University

Point-of-Care Molecular Diagnostics

Marco Schito, PhD, Henry Jackson Foundation, DAIDS, NIH, Bethesda, MD

New Approaches in HIV Testing

Moderator – Steven Ethridge, MT(ASCP), Centers for Disease Control and Prevention

ELISA Strength of Signal in a Protocol to Prevent Mother-to-Child HIV Transmission

Michael Loeffelholz, PhD, ABMM, University of Texas Medical Branch

Rapid Detection of HIV-1 p24 Antigen and of Antibodies to HIV-1 and HIV-2 Using Magnetic Immunochromatography

Timothy C. Granade, MS, Centers for Disease Control and Prevention

Europium Nanoparticle-based Immunoassay (ENIA) for Sensitive and Early Detection of Human Immunodeficiency Virus Type 1 Capsid (p24) Antigen

Shixing Tang, MD, PhD, Food and Drug Administration

A novel method of creating molecular control used in the NLHRS HIV-2 Viral Load Assay and subsequent results in a NIBSC-WHO study to evaluate candidate international standards for HIV-2 RNA

John Kim, PhD, National Laboratory for HIV Reference Services, Public Health Agency of Canada

Conference Wrap-Up & Next Steps

Moderated by Bernard Branson, MD, Centers for Disease Control and Prevention